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Project:
NIR hyperspectral imaging
Champions: Micheal
Huebschman , Sasidar Katari
We built a near-infrared
hyperspectral imaging system capable of detecting spectral signature
of samples emitting in the NIR range of 950-1300. The acquisition of
photoluminescence from dispersed single-walled nanotubes (SWNT) samples
demonstrated that the system sensitivity is sufficient to detect NIR
fluorescent material possessing quantum efficiencies <10%. In the
future, as more fluorophores that emit in the NIR are made available,
new applications using highly multiplexed labels can be conducted on
samples, including for example, the ability to image multiple immunohistochemistry
biomarkers below the surface in tissue.
Fig. 1 The
red and blue traces represent normalized photoluminescence spectra
of dispersed and aggregated SDDBS-SWNTs, respectively, acquired
with an exposure time of 4.5 s. The green trace represents a
normalized absorption spectrum of dispersed SDDBS-SWNTs where
the predominant semi-conducting SWNT structures are denoted by
(n,m) chiral indices. |
Fig. 2 (A)
NIR (950-1300 nm) image of SkBr3 cells (black regions) plated
against a fluorescent background of PbS quantum dots (white/gray
regions). The 10-µm (black) scale bar shown in (A) applies
to both images. (B) Visible image of DAPI-treated SkBr3 cells
(blue regions) acquired through the eyepiece of the microscope
using a 3 megapixel Sony digital camera, a 330-385 nm bandpass
filter, and a 420-nm longpass filter. The black lines and numbers
are from the eyepiece
reticle. |
Related Publications
"Fabrication
and evaluation of a near-infrared hyperspectral imaging system" Katari
S C, Wallack M.N. , Huebschman M., Pantono P.& Garner H.R.
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